Effects of Macromolecular Crowding on Protein Folding - in-vitro equilibrium and kinetic studies on selected model systems
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چکیده
............................................................................................ III LIST OF ABBREVIATIONS ...................................................................... VIII ENKEL SAMMANFATTNING PÅ SVENSKA ................................................ VI LIST OF PUBLICATIONS ........................................................................ VIII
منابع مشابه
Macromolecular crowding modulates folding mechanism of alpha/beta protein apoflavodoxin.
Protein dynamics in cells may be different from those in dilute solutions in vitro, because the environment in cells is highly concentrated with other macromolecules. This volume exclusion because of macromolecular crowding is predicted to affect both equilibrium and kinetic processes involving protein conformational changes. To quantify macromolecular crowding effects on protein folding mechan...
متن کاملMacromolecular crowding compacts unfolded apoflavodoxin and causes severe aggregation of the off-pathway intermediate during apoflavodoxin folding.
To understand how proteins fold in vivo, it is important to investigate the effects of macromolecular crowding on protein folding. Here, the influence of crowding on in vitro apoflavodoxin folding, which involves a relatively stable off-pathway intermediate with molten globule characteristics, is reported. To mimic crowded conditions in cells, dextran 20 at 30% (w/v) is used, and its effects ar...
متن کاملEffect of Hydration and Macromolecular Crowding on Peptide Conformation, Aggregation and Folding Kinetics
Protein folding/misfolding in vivo takes place in a highly crowded and confined environment. Such crowded environment can possibly lead to fewer water molecules surrounding a protein of interest than that seen under in vitro conditions wherein typically dilute aqueous solutions are used. When considering the aforesaid cellular characteristics, such as water depletion and macromolecular crowding...
متن کاملAbstract Submitted for the MAR11 Meeting of The American Physical Society Competition between chemical denaturation and macromolecular crowding effects on the folding dynamics of proteins1 ANTONIOS SAMIO- TAKIS,
Submitted for the MAR11 Meeting of The American Physical Society Competition between chemical denaturation and macromolecular crowding effects on the folding dynamics of proteins1 ANTONIOS SAMIOTAKIS, MARGARET CHEUNG, University of Houston — It is well known that proteins fold and function in the crowded environment of the cell’s interior. In the recent years it has been established that the so...
متن کاملProtein folding and binding in confined spaces and in crowded solutions.
Simple theoretical models are presented to illustrate the effects of spatial confinement and macromolecular crowding on the equilibria and rates of protein folding and binding. Confinement is expected to significantly stabilize the folded state, but for crowding only a marginal effect on protein stability is expected. In confinement the unfolded chain is restricted to a cage but in crowding the...
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تاریخ انتشار 2013